|
| Status |
Public on Feb 19, 2009 |
| Title |
GPR30-antisense Transfection, OHT Induction 1h, B |
| Sample type |
RNA |
| |
|
| Source name |
SKBR3
|
| Organism |
Homo sapiens |
| Characteristics |
Estrogen Receptor alpha and beta negative breast cancer cells
|
| Treatment protocol |
SKBR3 cells were transfected in 10 cm plates at roughly 60% density with Fugene for 30 hours (3microl/microgram). Cells were induced for 1 h with respective treatment.
|
| Growth protocol |
SKBR3 cells were grown in RPMI-1640 media supplemented with 10% FCS and L-Glutamine. Cells were switched to media without serum 24 h before transfection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TriZOL.
|
| Label |
biotin UTP
|
| Label protocol |
As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip, Illumina TotalPrep RNA Amplification Kit from Ambion, Catalog #IL1791
|
| |
|
| Hybridization protocol |
As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip
|
| Scan protocol |
As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip
|
| Description |
Gene expression analyses were carried out to identify genes regulated by 17-beta estradiol (E2) and Hydroxytamoxifen (OHT) through GPR30 in SKBR3 cells.
|
| Data processing |
Illumina Bead Studio, No background subtraction, No normalization
|
| |
|
| Submission date |
May 27, 2008 |
| Last update date |
Feb 19, 2009 |
| Contact name |
Deo Prakash Pandey |
| E-mail(s) |
Deo.Pandey@unige.ch
|
| Phone |
0041 22 3796811
|
| Fax |
0041 22 3796442
|
| URL |
http://www.picard.ch
|
| Organization name |
University of Geneva
|
| Department |
Cell Biology
|
| Street address |
30 Quai Ernest-Ansmert
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE11567 |
GPR30-mediated estrogen signaling in Estrogen Receptor alpha and beta negative SKBR3 breast cancer cells |
|
