|
Status |
Public on Feb 19, 2009 |
Title |
GPR30-antisense Transfection, OHT Induction 1h, C |
Sample type |
RNA |
|
|
Source name |
SKBR3
|
Organism |
Homo sapiens |
Characteristics |
Estrogen Receptor alpha and beta negative breast cancer cells
|
Treatment protocol |
SKBR3 cells were transfected in 10 cm plates at roughly 60% density with Fugene for 30 hours (3microl/microgram). Cells were induced for 1 h with respective treatment.
|
Growth protocol |
SKBR3 cells were grown in RPMI-1640 media supplemented with 10% FCS and L-Glutamine. Cells were switched to media without serum 24 h before transfection.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TriZOL.
|
Label |
biotin UTP
|
Label protocol |
As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip, Illumina TotalPrep RNA Amplification Kit from Ambion, Catalog #IL1791
|
|
|
Hybridization protocol |
As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip
|
Scan protocol |
As suggested by the manufacturer, Illumina for beadchip Illumina human-6 v2.0 expression beadchip
|
Description |
Gene expression analyses were carried out to identify genes regulated by 17-beta estradiol (E2) and Hydroxytamoxifen (OHT) through GPR30 in SKBR3 cells.
|
Data processing |
Illumina Bead Studio, No background subtraction, No normalization
|
|
|
Submission date |
May 27, 2008 |
Last update date |
Feb 19, 2009 |
Contact name |
Deo Prakash Pandey |
E-mail(s) |
Deo.Pandey@unige.ch
|
Phone |
0041 22 3796811
|
Fax |
0041 22 3796442
|
URL |
http://www.picard.ch
|
Organization name |
University of Geneva
|
Department |
Cell Biology
|
Street address |
30 Quai Ernest-Ansmert
|
City |
Geneva |
ZIP/Postal code |
1211 |
Country |
Switzerland |
|
|
Platform ID |
GPL6102 |
Series (1) |
GSE11567 |
GPR30-mediated estrogen signaling in Estrogen Receptor alpha and beta negative SKBR3 breast cancer cells |
|