|
| Status |
Public on Jan 07, 2009 |
| Title |
MCF7_FAIRE_1 |
| Sample type |
genomic |
| |
|
| Source name |
MCF7
|
| Organism |
Homo sapiens |
| Characteristics |
MCF7
|
| Growth protocol |
MCF7 cells were grown in DMEM and LNCaP cells in RPMI. Media were supplemented with 10% FBS.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were cross-linked with 1% formaldehyde and processed as in (Nagy et al. 2003; Hogan et al. 2006)
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Scanned at the Dana-Farber microarray core facility
|
| Description |
Chromatin structure was analyzed in MCF7 and LNCaP cells using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) (Nagy et al. 2003; Hogan et al. 2006)
|
| Data processing |
Model-based analysis of tiling array (MAT), chip.dfci.harvard.edu/~wli/MAT/
|
| |
|
| Submission date |
May 28, 2008 |
| Last update date |
Jan 07, 2009 |
| Contact name |
Jérôme Eeckhoute |
| E-mail(s) |
jerome.eeckhoute@inserm.fr
|
| Organization name |
CNRS
|
| Lab |
INSERM UMR 1011, Université Lille-Nord de France
|
| Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
|
| City |
Lille |
| ZIP/Postal code |
59045 |
| Country |
France |
| |
|
| Platform ID |
GPL4915 |
| Series (1) |
|
