|
Status |
Public on Jan 07, 2009 |
Title |
MCF7_FAIRE_2 |
Sample type |
genomic |
|
|
Source name |
MCF7
|
Organism |
Homo sapiens |
Characteristics |
MCF7
|
Growth protocol |
MCF7 cells were grown in DMEM and LNCaP cells in RPMI. Media were supplemented with 10% FBS.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cells were cross-linked with 1% formaldehyde and processed as in (Nagy et al. 2003; Hogan et al. 2006)
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Scanned at the Dana-Farber microarray core facility
|
Description |
Chromatin structure was analyzed in MCF7 and LNCaP cells using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) (Nagy et al. 2003; Hogan et al. 2006)
|
Data processing |
Model-based analysis of tiling array (MAT), chip.dfci.harvard.edu/~wli/MAT/
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|
|
Submission date |
May 28, 2008 |
Last update date |
Jan 07, 2009 |
Contact name |
Jérôme Eeckhoute |
E-mail(s) |
jerome.eeckhoute@inserm.fr
|
Organization name |
CNRS
|
Lab |
INSERM UMR 1011, Université Lille-Nord de France
|
Street address |
Bâtiment J&K, Faculté de Médecine de Lille-Pôle Recherche, Boulevard du Professeur Leclerc
|
City |
Lille |
ZIP/Postal code |
59045 |
Country |
France |
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|
Platform ID |
GPL4915 |
Series (1) |
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