|
| Status |
Public on Jan 31, 2018 |
| Title |
RE8 |
| Sample type |
RNA |
| |
|
| Source name |
plasma from child who was suffered relapse after chemotherapy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: plasma from child who was suffered relapse after chemotherapy
|
| Treatment protocol |
The blood samples were stored in 4℃. Centifuge at the speed of 1500rpm/min for 10 minutes at 4℃ and repeat the procedure once. The plasma was stored at -80℃.
|
| Growth protocol |
The plasma that was seperated from the blood in 8 hours from the blood drawing,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol exaction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
TaqMan®
|
| Label protocol |
The reverse of RNA was performed using the TaqMan MicroRNA Reverse Transcription Kit following the Manufacturer’s instructions. Quantitative real-time PCR were performed (7900HT, ABI) with 12 cycles at 95℃ for 15 seconds and 60℃ for 4minutes. And hold at 99.9℃ for 10 minutes.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Data processing |
（SDS2.4）RQ Manager. The original ct values of the plasma miRNAs were normalized using the control miRNA: U6.
The Ct value is the number of PCR cycles when the fluorescence value reaches the threshold, which is a parameter without unit. The Ct value has a linear relationship with the logarithm of the original template copy number, and the more the initial copy number, the smaller the Ct value.
|
| |
|
| Submission date |
Jan 30, 2018 |
| Last update date |
Jan 31, 2018 |
| Contact name |
Qian Jiang |
| E-mail(s) |
jqbest06@163.com
|
| Phone |
+8618810254671
|
| Organization name |
Beijing Childrens' Hospital Capital Medical University
|
| Department |
Hematology Oncology Centre
|
| Street address |
No.56 Nanlishi Road Xicheng District Beijing
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
010 |
| Country |
China |
| |
|
| Platform ID |
GPL24558 |
| Series (1) |
| GSE109868 |
Expression data of plasma microRNA from the healthy children and children who diagnosed with acute lymphoblastic leukemia |
|
