|
Status |
Public on Jan 31, 2020 |
Title |
Patient 42, sample B100, Sigmoid colon |
Sample type |
genomic |
|
|
Source name |
Sigmoid colon
|
Organism |
Homo sapiens |
Characteristics |
disease status: Synchronous colorectal cancer (SCRC) patient pair number: 42 Sex: Male age at diagnosis: 61 tissue: Sigmoid colon
|
Treatment protocol |
Not applicable.
|
Growth protocol |
Not applicable.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNAs were extracted according to manufacturer's protocols.
|
Label |
Biotin
|
Label protocol |
MIP probe annealing and hybridization were performed according to manufacturer's protocols.
|
|
|
Hybridization protocol |
The digested DNA target was hybridized onto the OncoScan array and incubated at 49ºC in a GeneChip Hybridization oven 640 (Affymetrix) for 17h at 60rpm. OncoScan arrays were then washed and stained in a GeneChips Fluidics Station 450 (Affymetrix) using OncoScan stain and wash reagents according to the manufacturer’s instructions.
|
Scan protocol |
The microarrays were finally scanned with a GeneChip scanner 3000 (Affymetrix).
|
Description |
Pac42_B100
|
Data processing |
CEL files were processed using the Affymetrix OncoScan console (version 1.3) and the Affymetrix ChAS software (version 3.2.0.1252).
|
|
|
Submission date |
Feb 01, 2018 |
Last update date |
Jan 31, 2020 |
Contact name |
Juan Luis Garcia |
E-mail(s) |
jlgarcia@usal.es
|
Phone |
+34923294812
|
Organization name |
Centro de Investigación del Cáncer IBMCC-CIC
|
Lab |
Laboratorio 12
|
Street address |
Campus Miguel de Unamuno
|
City |
Salamanca |
State/province |
Salamanca |
ZIP/Postal code |
37007 |
Country |
Spain |
|
|
Platform ID |
GPL21558 |
Series (1) |
GSE110026 |
Redefining synchronous colorectal cancer on the basis of clonality |
|