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Sample GSM298225 Query DataSets for GSM298225
Status Public on Jul 13, 2009
Title small airways, non-smoker 065
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 49
sex: M
ethnic group: black
smoking status: non-smoker
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description none
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 13, 2008
Last update date Nov 12, 2009
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (11)
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE11906 Quality Control in Microarray Assessment of Gene Expression in Human Airway Epithelium
GSE11952 Coordinate Control of Nrf2 Mediated Genes in the Human Small Airway Epithelium Highly Responsive to Smoking

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 431.082 P 0.000445901
AFFX-BioB-M_at 757.762 P 4.42873e-05
AFFX-BioB-3_at 428.671 P 7.00668e-05
AFFX-BioC-5_at 1317.83 P 6.02111e-05
AFFX-BioC-3_at 1881.96 P 5.16732e-05
AFFX-BioDn-5_at 3904.58 P 4.42873e-05
AFFX-BioDn-3_at 8195.17 P 7.00668e-05
AFFX-CreX-5_at 18722.9 P 5.16732e-05
AFFX-CreX-3_at 22290.8 P 4.42873e-05
AFFX-DapX-5_at 7.3291 A 0.724854
AFFX-DapX-M_at 16.6183 A 0.631562
AFFX-DapX-3_at 2.631 A 0.998752
AFFX-LysX-5_at 16.1643 A 0.617401
AFFX-LysX-M_at 50.7635 A 0.41138
AFFX-LysX-3_at 49.5486 A 0.165861
AFFX-PheX-5_at 6.10667 A 0.953518
AFFX-PheX-M_at 8.01071 A 0.760937
AFFX-PheX-3_at 9.68126 A 0.868639
AFFX-ThrX-5_at 9.24933 A 0.843268
AFFX-ThrX-M_at 51.1166 A 0.216524

Total number of rows: 54675

Table truncated, full table size 1639 Kbytes.




Supplementary file Size Download File type/resource
GSM298225.CEL.gz 8.5 Mb (ftp)(http) CEL
GSM298225.CHP.gz 13.6 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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