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Sample GSM298227 Query DataSets for GSM298227
Status Public on Jul 13, 2009
Title small airways, non-smoker 076
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 29
sex: M
ethnic group: hispanic
smoking status: non-smoker
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description none
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 13, 2008
Last update date Aug 28, 2018
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (13)
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE11906 Quality Control in Microarray Assessment of Gene Expression in Human Airway Epithelium
GSE11952 Coordinate Control of Nrf2 Mediated Genes in the Human Small Airway Epithelium Highly Responsive to Smoking
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 369.727 P 0.000662269
AFFX-BioB-M_at 864.471 P 4.42873e-05
AFFX-BioB-3_at 394.243 P 0.000126798
AFFX-BioC-5_at 1262.86 P 5.16732e-05
AFFX-BioC-3_at 1802.03 P 4.42873e-05
AFFX-BioDn-5_at 2641.57 P 4.42873e-05
AFFX-BioDn-3_at 6914.23 P 6.02111e-05
AFFX-CreX-5_at 19036 P 5.16732e-05
AFFX-CreX-3_at 21346.5 P 4.42873e-05
AFFX-DapX-5_at 17.015 A 0.368438
AFFX-DapX-M_at 7.36676 A 0.737173
AFFX-DapX-3_at 5.42631 A 0.945802
AFFX-LysX-5_at 32.5224 A 0.156732
AFFX-LysX-M_at 21.1608 A 0.631573
AFFX-LysX-3_at 30.5753 P 0.0309764
AFFX-PheX-5_at 4.49634 A 0.843268
AFFX-PheX-M_at 10.0824 A 0.659339
AFFX-PheX-3_at 26.9061 A 0.313723
AFFX-ThrX-5_at 3.5999 A 0.852082
AFFX-ThrX-M_at 4.5202 A 0.843268

Total number of rows: 54675

Table truncated, full table size 1646 Kbytes.




Supplementary file Size Download File type/resource
GSM298227.CEL.gz 8.3 Mb (ftp)(http) CEL
GSM298227.CHP.gz 13.5 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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