|
| Status |
Public on Feb 08, 2018 |
| Title |
8hrDT.D7.DTR+.F4-80+.MHCII-#3 |
| Sample type |
RNA |
| |
|
| Source name |
Muscle
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57Bl6
genotype/variation: Foxp3.DTR+
age: 12 Weeks
Sex: Male
injury: Cardiotoxin
time point (days after injury): 7
cell type: muscle macrophages
phenotype markers: Ly6g- SiglecF- CD11b+ F4-80+ MHCII-
separation method: Dissection / Trimming
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling and Controls Kit.
|
| |
|
| Hybridization protocol |
Ambion WT Expression Kit.
|
| Scan protocol |
Affymetrix GeneChip Scanner.
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Feb 07, 2018 |
| Last update date |
Feb 08, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE110308 |
Treg cells limit IFN-g production to control macrophage accrual and phenotype during skeletal muscle regeneration |
|
