|
| Status |
Public on Jun 13, 2018 |
| Title |
activin/RA-treated AC_ERK3 MO1/2 |
| Sample type |
RNA |
| |
|
| Source name |
activin/RA-treated ACs injected with ERK3 MO1/2, stage 15
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: animal cap
developmental stage: 15
injected mo: ERK3 MO1/2
treatment: activin/RA
|
| Treatment protocol |
Control MO (80 ng), ERK3 MO1/2 (40 ng each of MO1 and MO2), or ERK3 MO3 (80 ng) were injected into the animal regions of all blastomeres at the 4-cell stage. The animal caps were dissected from the injected embryos at stage 9, cultured alone or in the presence of activin plus retinoic acid (activin/RA), and harvested at stage 15.
|
| Growth protocol |
Xenopus laevis embryos were obtained by in vitro fertilization and cultured in 0.1x MBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 250 ng total RNA using the GeneChip 3’ IVT Express Kit (Affymetrix) according to the manufacturer’s instruction.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Xenopus laevis Genome 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
| Description |
Kidney_AB_15
ERK3 MO1/2 (40 ng each of MO1 and MO2) were injected into the animal regions of all blastomeres at the 4-cell stage. The animal caps were dissected from the injected embryos at stage 9, cultured in the presence of activin plus retinoic acid (activin/RA), and harvested at stage 15.
|
| Data processing |
The data were analyzed with GeneSpring GX (Agilent Technologies).
|
| |
|
| Submission date |
Feb 09, 2018 |
| Last update date |
Jun 13, 2018 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10756 |
| Series (2) |
| GSE110427 |
ERK3 is essential for establishment of epithelial architecture [ERK3 KD] |
| GSE110429 |
ERK3 is essential for establishment of epithelial architecture |
|
