|
Status |
Public on Feb 09, 2021 |
Title |
Con-Ad-1 |
Sample type |
SRA |
|
|
Source name |
mouse lung alveolar epithelial type 2 cells
|
Organism |
Mus musculus |
Characteristics |
cell type: lung alveolar epithelial type 2 cells strain: C57BL/6 treatment: Con adenovirus
|
Treatment protocol |
The newly isolated AEC2s were infected with Con or Slug adenovirus for 48 hr.
|
Growth protocol |
The newly isolated AEC2s were cultured on Fibronectin-coated dishes with DMEM containing 10 % FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) according to manufacturers instructions. 1-2 g of total RNA was subjected to library synthesis using the KAPA Stranded RNA-Seq Library Prep Kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
Con-Ad infected AEC2s
|
Data processing |
Solexa pipeline version 1.8, FastQC, Hisat2, and StringTie software used for basecalling. Read quality was checked for each sample using FastQC v0.10.1. High-quality reads were then imported into Hisat2 for alignment. Using StringTie and R software Ballgown to get FPKM value.
Genome_build: mm10 Supplementary_files_format_and_content: Tab-delimited text file includes FPKMs (log2-transformed with an offset value of 1.0) for each sample.
|
|
|
Submission date |
Feb 09, 2018 |
Last update date |
Feb 09, 2021 |
Contact name |
xiaoxi lv |
E-mail(s) |
lvxiaoxi@imm.ac.cn
|
Organization name |
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
|
Street address |
No Jia2, Nanwei road, Xi Cheng district
|
City |
Beijing |
ZIP/Postal code |
100050 |
Country |
China |
|
|
Platform ID |
GPL21103 |
Series (1) |
GSE110430 |
Slug-Ad infected mouse lung alveolar type 2 cells |
|
Relations |
BioSample |
SAMN08514602 |
SRA |
SRX3678964 |