|
| Status |
Public on Feb 09, 2021 |
| Title |
Con-Ad-2 |
| Sample type |
SRA |
| |
|
| Source name |
mouse lung alveolar epithelial type 2 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: lung alveolar epithelial type 2 cells
strain: C57BL/6
treatment: Con adenovirus
|
| Treatment protocol |
The newly isolated AEC2s were infected with Con or Slug adenovirus for 48 hr.
|
| Growth protocol |
The newly isolated AEC2s were cultured on Fibronectin-coated dishes with DMEM containing 10 % FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) according to manufacturers instructions.
1-2 g of total RNA was subjected to library synthesis using the KAPA Stranded RNA-Seq Library Prep Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Con-Ad infected AEC2s
|
| Data processing |
Solexa pipeline version 1.8, FastQC, Hisat2, and StringTie software used for basecalling.
Read quality was checked for each sample using FastQC v0.10.1.
High-quality reads were then imported into Hisat2 for alignment.
Using StringTie and R software Ballgown to get FPKM value.
Genome_build: mm10
Supplementary_files_format_and_content: Tab-delimited text file includes FPKMs (log2-transformed with an offset value of 1.0) for each sample.
|
| |
|
| Submission date |
Feb 09, 2018 |
| Last update date |
Feb 09, 2021 |
| Contact name |
xiaoxi lv |
| E-mail(s) |
lvxiaoxi@imm.ac.cn
|
| Organization name |
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
No Jia2, Nanwei road, Xi Cheng district
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE110430 |
Slug-Ad infected mouse lung alveolar type 2 cells |
|
| Relations |
| BioSample |
SAMN08514603 |
| SRA |
SRX3678965 |
