|
| Status |
Public on Feb 05, 2019 |
| Title |
H550 |
| Sample type |
RNA |
| |
|
| Source name |
Primary diffuse large B-cell lymphoma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: R-CHOP
ipi: 3
|
| Treatment protocol |
The tumor sample was after removal snap frozen and stored in OCT at -80ºC. From each OCT block, a small fraction of the sample was removed using a scalpel, washed twice in PBS, and homogenized using the TissueLyser II (Qiagen).
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a modified protocol combining Trizol reagent (Invitrogen) and mirVana microRNA isolation kit (Ambion/ThermoFisher Scientific)
|
| Label |
Biotin
|
| Label protocol |
Combination of WT Expression Kit (Ambion) and Affymetrix GeneChip WT Terminal Labeling Kit
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Exon expression data from diffuse large B-cell lymphoma samples
|
| Data processing |
Data were analyzed using Partek Genomics Suite version 6.6. The .CEL-files were pre-processed using the Robust Multichip Average procedure (RMA) as implemented in the Partek software. The .CEL files were analyzed by the alternative splice analysis of variance (asANOVA) incorporated in Partek Genomics.
HuEx-1_0-st-v2.pgf
HuEx-1_0-st-v2.mpf
|
| |
|
| Submission date |
Feb 13, 2018 |
| Last update date |
Feb 05, 2019 |
| Contact name |
Julie Støve Bødker |
| E-mail(s) |
j.boedker@rn.dk
|
| Organization name |
Aalborg University Hospital
|
| Department |
Department of Heamatology
|
| Lab |
Research Laboratory
|
| Street address |
Sdr. Skovvej 15
|
| City |
Aalborg |
| ZIP/Postal code |
9000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE110548 |
Exon expression profiling of de novo diffuse large B-cell lymphoma samples |
|
