|
| Status |
Public on Oct 27, 2021 |
| Title |
BKO408 2 (BLNK1) |
| Sample type |
RNA |
| |
|
| Source name |
preB-ALL cell line developed in Blnk KO mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: preB-ALL cell line BKO408
developed in: Blnk KO mice
|
| Treatment protocol |
No treatment was provided.
|
| Growth protocol |
BKO408 cells were grown in RPMI1640 culture medium supplemented with 10% fetal bovine serum, and 5 ng/ml IL-7. Mouse ALL developed in Blnk homozygous knock-out, Cebpb LAP transgenic, or Blnk KO/LAP tg mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using RNA Easy kit (QIAGEN) according to the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 7.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 PM Array. GeneChips were washed and stained in the Affymetrix GeneAtlas Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneAtlas Imaging Station.
|
| Description |
Gene expression data from mouse ALL
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Feb 16, 2018 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Takuro Nakamura |
| E-mail(s) |
takuro-ind@umin.net
|
| Phone |
81-3-3570-0462
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
The Cancer Institute
|
| Lab |
Carcinogenesis
|
| Street address |
3-8-31 Ariake, Koto-ku
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11180 |
| Series (2) |
| GSE110737 |
C/EBPb cooperates with BLNK mutation in preB leukemogenesis (BLNK1) |
| GSE110739 |
C/EBPb cooperates with BLNK mutation in preB leukemogenesis |
|
