|
| Status |
Public on Apr 26, 2018 |
| Title |
Liver_Sedentary_Control_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver tissue from sedentary mice fed with control diet
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6N
age: 11-12 weeks
gender: Male
tissue: Liver
diet: control
exercise: sedentary
|
| Treatment protocol |
As described in the paper. Briefly, mice were assigned to either a diet supplemented with 100 mg/kg vitamin C and 2,000 IU/kg vitamin E (as α-tocopheryl acetate) or to control diet (0 mg/kg vitamin C, 149 IU/kg vitamin E) based on the purified diet C1000 (Altromin, Lage, Germany). After 4 weeks of feeding, at an age of 11-12 weeks, the animals were subjected to an acute bout of exercise (60 min at 13 m/min and 14° inclination) on a treadmill as described previously (Hoene et al., 2010). Mice remaining in their cages, but in close proximity to the treadmill, served as controls and were deprived of food for the last 60 min before they were killed, to align with the feeding state of the exercised group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Fibrous Tissue Kit (Qiagen) including DNase digestion.
|
| Label |
biotin
|
| Label protocol |
Ovation Pico WTA System V2 and Encore Biotin Module (Nugen).
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit) including minor changes as suggested by the Encore Biotin protocol.
|
| Scan protocol |
According to the Affymetrix expression protocol (GeneChip Scanner 3000 7G).
|
| Description |
Sed_Ctrl_2402
Gene expression data from liver tissue from sedentary mice fed with control diet, biological replicate 2.
|
| Data processing |
RMA probeset summary with standard settings of the Affymetrix Expression Console software (build 1.4.1.46) including median polish and Sketch-Quantile normalisation.
|
| |
|
| Submission date |
Feb 16, 2018 |
| Last update date |
Apr 26, 2018 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE110747 |
A vitamin E- supplemented antioxidant diet interferes with the acute adaptation of the liver to physical exercise in mice |
|
