|
Status |
Public on Jun 21, 2020 |
Title |
MSC2 untreated, biological rep1 |
Sample type |
RNA |
|
|
Source name |
immortalized MSC2 cell line
|
Organism |
Mus musculus |
Characteristics |
strain/background: BALB/c syngeneic cell line: MSC2 cell type: immortalized MDSC cell line experimental condition: untreated
|
Treatment protocol |
MSC2 were treated with IL-4 (100ng/ml, peprotech) or left untreated. 4 days after treatment initiation, cells were washed twice with PBS and pellet was used for RNA extraction.
|
Growth protocol |
5e5 MSC2 were incubated for 4 days on a T75 flask in 20ml RPMI (invitrogen) containing 10% of heat inactivated FCS, L-Glutamine (2mM), sodium pyruvate (1mM), streptomycin (150U/ml), and penicillin (200U/ml) in the presence or in the absence of IL4 (100ng/ml, peprotech).
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer guidelines, with minor modifications. Extraction was followed by Qiagen RNeasy column clean-up according to manufacturer instructions.
|
Label |
biotin
|
Label protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
|
|
Hybridization protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
Scan protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
Description |
MSC2_ctr_rep1 Gene expression profile of MSC2 cells.
|
Data processing |
RMA of .CEL files using Bioconductor affy library and Brainarray custom CDF MGU74Av2_Mm_ENTREZG_21.0.0. In RMA, PM values have been background adjusted, normalized using quantile normalization, and expression measure calculated using median polish summarization.
|
|
|
Submission date |
Feb 18, 2018 |
Last update date |
Jun 21, 2020 |
Contact name |
Silvio Bicciato |
E-mail(s) |
silvio.bicciato@unipd.it
|
Phone |
+39-049-827-6108
|
Organization name |
University of Padova
|
Department |
Molecular Medicine
|
Street address |
via U. Bassi 59/b
|
City |
Padova |
ZIP/Postal code |
35131 |
Country |
Italy |
|
|
Platform ID |
GPL24620 |
Series (2) |
GSE110773 |
Expression data from MSC2 cells treated with IL4 |
GSE110774 |
Expression data from myeloid-derived suppressor cells (MDSCs) |
|