|
| Status |
Public on Jun 21, 2020 |
| Title |
MSC2+IL4, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
immortalized MSC2 cell line treated with IL4
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: BALB/c syngeneic
cell line: MSC2
cell type: immortalized MDSC cell line
experimental condition: IL4 (100ng/ml for 4 days)
|
| Treatment protocol |
MSC2 were treated with IL-4 (100ng/ml, peprotech) or left untreated. 4 days after treatment initiation, cells were washed twice with PBS and pellet was used for RNA extraction.
|
| Growth protocol |
5e5 MSC2 were incubated for 4 days on a T75 flask in 20ml RPMI (invitrogen) containing 10% of heat inactivated FCS, L-Glutamine (2mM), sodium pyruvate (1mM), streptomycin (150U/ml), and penicillin (200U/ml) in the presence or in the absence of IL4 (100ng/ml, peprotech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer guidelines, with minor modifications. Extraction was followed by Qiagen RNeasy column clean-up according to manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
| |
|
| Hybridization protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
| Scan protocol |
According to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004.
|
| Description |
MSC2_IL4_rep2
Gene expression profile of MSC2 cells treated with IL4 for 4 days.
|
| Data processing |
RMA of .CEL files using Bioconductor affy library and Brainarray custom CDF MGU74Av2_Mm_ENTREZG_21.0.0. In RMA, PM values have been background adjusted, normalized using quantile normalization, and expression measure calculated using median polish summarization.
|
| |
|
| Submission date |
Feb 18, 2018 |
| Last update date |
Jun 21, 2020 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL24620 |
| Series (2) |
| GSE110773 |
Expression data from MSC2 cells treated with IL4 |
| GSE110774 |
Expression data from myeloid-derived suppressor cells (MDSCs) |
|
