|
| Status |
Public on Feb 20, 2018 |
| Title |
Flt3 KO cDC2, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Flt3 KO mouse spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
cell type: cDC2
genotype: Flt3 KO
|
| Treatment protocol |
Mice were analyzed at steady-state
|
| Growth protocol |
Single cells supsensions were made from mouse spleens by digestion with Collagenase B and DNAse 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Digestion of spleens with Collagenase B and DNAse 1
|
| Label |
Biotin
|
| Label protocol |
Ambion WT Expression kit, Genechip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Hybed onto Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
Not provided
|
| Data processing |
Data was analyzed using DNASTAR Arraystar software
|
| |
|
| Submission date |
Feb 19, 2018 |
| Last update date |
Feb 20, 2018 |
| Contact name |
Vivek Durai |
| E-mail(s) |
duraiv@wustl.edu
|
| Organization name |
Washington University in St. Louis School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Kenneth M. Murphy
|
| Street address |
4940 Parkview Place, Dock at CSRB, Murphy Lab 7766
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE110789 |
Gene expression in WT, Flt3 KO, and Flt3L KO cDC1s and cDC2s |
| GSE110812 |
Altered compensatory cytokine signaling underlies the discrepancy between Flt3-/- and Flt3l-/- mice |
|
