|
Status |
Public on Feb 20, 2018 |
Title |
Flt3 KO cDC2, biological rep2 |
Sample type |
RNA |
|
|
Source name |
Flt3 KO mouse spleen
|
Organism |
Mus musculus |
Characteristics |
tissue: spleen cell type: cDC2 genotype: Flt3 KO
|
Treatment protocol |
Mice were analyzed at steady-state
|
Growth protocol |
Single cells supsensions were made from mouse spleens by digestion with Collagenase B and DNAse 1
|
Extracted molecule |
total RNA |
Extraction protocol |
Digestion of spleens with Collagenase B and DNAse 1
|
Label |
Biotin
|
Label protocol |
Ambion WT Expression kit, Genechip WT Terminal Labeling and Controls Kit
|
|
|
Hybridization protocol |
Hybed onto Mouse Gene 1.0 ST Arrays (Affymetrix)
|
Scan protocol |
Not provided
|
Data processing |
Data was analyzed using DNASTAR Arraystar software
|
|
|
Submission date |
Feb 19, 2018 |
Last update date |
Feb 20, 2018 |
Contact name |
Vivek Durai |
E-mail(s) |
duraiv@wustl.edu
|
Organization name |
Washington University in St. Louis School of Medicine
|
Department |
Pathology and Immunology
|
Lab |
Kenneth M. Murphy
|
Street address |
4940 Parkview Place, Dock at CSRB, Murphy Lab 7766
|
City |
Saint Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE110789 |
Gene expression in WT, Flt3 KO, and Flt3L KO cDC1s and cDC2s |
GSE110812 |
Altered compensatory cytokine signaling underlies the discrepancy between Flt3-/- and Flt3l-/- mice |
|