|
| Status |
Public on Feb 27, 2018 |
| Title |
d5il10_cebp |
| Sample type |
SRA |
| |
|
| Source name |
iBAd Cell Line
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
genotype: IL10Ra-Expressing
stimulus: differentiated, treated with IL10
chip antibody: anti-CEBPB
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Purified nuclei from iBAD cells were lysed and chromatin was sheared using a Bioruptor prior to immunoprecipitation with anti-CEBPB antibodies
Libraries were constructed using Kapa's LTP Library Preparation kit according to manufactuer's instruction.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Reads were aligned to the mouse genome (NCBI37/mm9) with Bowtie2. Uniquely mapped reads were used for peak calling and annotation using HOMER (Heinz et al., 2010). Peaks were called if they passed a false discovery rate of 0.01 and were enriched over input. Peaks were annotated to the nearest TSS.
Genome_build: mm9
Supplementary_files_format_and_content: bedGraph files for each sample represent the read coverage at each genomic position
|
| |
|
| Submission date |
Feb 27, 2018 |
| Last update date |
Feb 28, 2018 |
| Contact name |
Peter Tontonoz |
| Organization name |
UCLA
|
| Department |
Pathology
|
| Lab |
Tontonoz Lab
|
| Street address |
675 Charles E Young
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE94654 |
IL-10 signaling remodels adipose chromatin architecture to limit thermogenesis and energy expenditure |
| GSE111208 |
IL-10 signaling remodels adipose chromatin architecture to limit thermogenesis and energy expenditure [ChIP-Seq] |
|
| Relations |
| BioSample |
SAMN08618430 |
| SRA |
SRX3747734 |
