|
| Status |
Public on Nov 14, 2018 |
| Title |
Strain U04 rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
Exponential phase yeast cultures, YNBG10
|
| Organism |
Clavispora lusitaniae |
| Characteristics |
strain: U04
|
| Growth protocol |
Overnight cultures grown in YPD were diluted into YNBG10 and grown at 30 C with shaking to mid-exponential phase, diluted into prewarmed medium then grown to mid-exponential phase before harvesting RNA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested from snap-frozen pellets (using liquid nitrogen) using the MasterPure Yeast RNA Purification Kit (Epicentre)
Libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (NeoPrep) following standar Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Fluconazole resistant
|
| Data processing |
Raw reads were aligned to genome (ATCC 42720) with gsnap (2018-02-05) spliced alignment
sam to bam conversion with picardtools (2.14.1)
bam to read counts using "featureCounts" from subread (1.6.0)
Genome_build: Broad v1
|
| |
|
| Submission date |
Mar 05, 2018 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Jason E Stajich |
| E-mail(s) |
jason.stajich@ucr.edu
|
| Organization name |
University of California, Riverside
|
| Department |
Microbiology & Plant Pathology
|
| Street address |
900 University Ave
|
| City |
Riverside |
| State/province |
CA - California |
| ZIP/Postal code |
92521 |
| Country |
USA |
| |
|
| Platform ID |
GPL24692 |
| Series (1) |
| GSE111421 |
Heterogeneous drug resistance in and antifungal naïve chronic infection |
|
| Relations |
| BioSample |
SAMN08636513 |
| SRA |
SRX3765673 |
