|
| Status |
Public on May 01, 2018 |
| Title |
SCC9_parental_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Parental SCC9 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cisplatin-resistance: No
|
| Treatment protocol |
SCC9 cells were treated with cisplatin (Sigma, St Louis, MO, USA) with concentrations that gradually increased from 10^-7 M to 10^-5 M.
|
| Growth protocol |
SCC9 and SCC9-res cells were cultured in DMEM-F12 (Gibco) supplemented with 10% foetal bovine serum and 400 ng/ml hydrocortisone (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted by Trizol Reagent (Life Technologies) according to manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Double-stranded cDNA was Cy3 labeled.
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized to Arraystar Human lncRNA array version 1 lncRNA expression microarray in NimbleGen Hybridization System.
|
| Scan protocol |
After hybridization and washing, the processed slides were scanned with the Axon GenePix 4000B microarray scanner.
|
| Description |
Non
|
| Data processing |
Raw data were extracted as paired files by NimbleScan software (version 2.5), which offers quantile normalization and background correction. Files of probe level (*_norm_RMA.pair) and gene summary (*_RMA.calls) were produced
|
| |
|
| Submission date |
Mar 08, 2018 |
| Last update date |
May 01, 2018 |
| Contact name |
Bodu Liu |
| E-mail(s) |
liuleopold@gmail.com
|
| Phone |
862039943500
|
| Organization name |
Sun Yat-sen University
|
| Street address |
School of Life Sciences, Sun Yat-sen U East Camp, High. Edu. Meg. Cntr.
|
| City |
Guangzhou |
| State/province |
State... |
| ZIP/Postal code |
510006 |
| Country |
China |
| |
|
| Platform ID |
GPL14715 |
| Series (1) |
| GSE111585 |
LncRNA and mRNA expression of cisplatin-resistant versus parental SCC9 cells |
|
