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Sample GSM3039416 Query DataSets for GSM3039416
Status Public on Mar 14, 2018
Title Testis from Prss54KO mouse_rep2
Sample type RNA
Source name Testis_Prss54KO mouse
Organism Mus musculus
Characteristics strain background: mixed 129Sv/C57BL/6
genotype/variation: Prss54KO
gender: male
age: 6-8 weeks
tissue: testis
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer's recommendations and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies, Santa Clara, CA, US) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).
Hybridization protocol Each slide was hybridized with 1.65ug Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions.
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=5μm, PMT 100%, 10%, 16bit.
Description Prss54ko2L testis
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, GeneSpring Software 12.6.1 (Agilent technologies, Santa Clara, CA, US).
Submission date Mar 13, 2018
Last update date Mar 15, 2018
Contact name Xuan Shang
Phone 15000081962
Organization name Shanghai Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Department Research Center for Experimental Medicine
Street address No.197, Ruijin 2nd Road, Huangpu District, Shanghai
City shanghai
State/province shanghai
ZIP/Postal code 200025
Country China
Platform ID GPL7202
Series (1)
GSE111758 Identification differentially expressed genes in the mouse testis caused by Prss54 deficiency

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 14.600567
DarkCorner 3.54877
A_52_P616356 3.172819
A_52_P580582 7.006638
A_52_P403405 15.706477
A_52_P819156 8.040783
A_51_P331831 6.8346214
A_51_P430630 3.1913083
A_52_P502357 3.1940656
A_52_P299964 3.1979585
A_51_P356389 3.20211
A_52_P684402 13.002489
A_51_P414208 8.686056
A_51_P280918 10.3895035
A_52_P613688 6.4677696
A_52_P258194 11.930099
A_52_P229271 6.7574115
A_52_P214630 9.376856
A_52_P579519 15.734524
A_52_P979997 3.2494862

Total number of rows: 41267

Table truncated, full table size 907 Kbytes.

Supplementary file Size Download File type/resource
GSM3039416_Prss54_2_L_251486840911_S01_GE1_107_Sep09_1_2.txt.gz 9.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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