|
| Status |
Public on Mar 20, 2018 |
| Title |
Wildtype_GMP_2 |
| Sample type |
RNA |
| |
|
| Source name |
Wildtype Bone Marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
cell type abbreviation: GMP
cell type full: Granulocyte Macrophage Progenitor
antibodies: Lin- Sca-1- c-Kit+ CD34+ FcR+
tissue: Bone Marrow
genotype: Wildtype
|
| Treatment protocol |
Bone marrow was collected from femurs and tibiae. Peripheral blood was collected from the retro-orbital sinus into microtainer tubes containing EDTA. Cells were purified using monoclonal monoclonal antibodies and then isolated on a fluorescence-activated cell sorter.
|
| Growth protocol |
All cells were purified from mice between 7 and 12 weeks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Wildtype GMP
sample name in non-normalized data file:
iltp2455-25
|
| Data processing |
Normalized data was restricted to a curated selection of probes that were supported by an Ensembl transcript and that were found on both V1.1 and V2.0 beadchips. The dataset was normalized with the normexp background correction followed by quantile normalization
|
| |
|
| Submission date |
Mar 19, 2018 |
| Last update date |
Mar 20, 2018 |
| Contact name |
Carolyn A de Graaf |
| E-mail(s) |
degraaf@wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE112010 |
Transcriptional profiling of eosinophil subsets in Interleukin-5 transgenic mice |
|
