|
| Status |
Public on May 22, 2018 |
| Title |
Spleen of non-infected Balb/c mice (Ba-nr2: MODEL) |
| Sample type |
RNA |
| |
|
| Source name |
SPLEEN
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
|
| Treatment protocol |
24 out of the 47 mice were infected with 10^6 stationary-phase L. infantum promastigotes via tail vein.
|
| Growth protocol |
47 BALB/c (purchased to Charles River Laboratories, France) mice (14-15 weeks old) were used in this study. Mice were randomly separated in two groups: (i) 23 control mice and (ii) 24 mice that were infected with 10^6 stationary-phase L. infantum promastigotes via tail vein. Mice were euthanized by cervical dislocation and spleens were removed and immediately stored in RNAlater at -70ºC (Sigma-Aldrich, St. Louis, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation from spleens (9-11 mg) was performed by cell disruption using FastPrep® System (ProScientific, Cedex, France) and Lysing Matrix D (MP Biomedicals, Solon, USA) in TRI-Reagent (Sigma-Aldrich, St. Louis, USA). RNeasy Mini Kit (Qiagen) was subsequently used for mRNA enrichment following manufacturer’s instructions
|
| Label |
FAM
|
| Label protocol |
The reverse transcription was performed with 2 ug of RNA with random hexamers using the High Capacity cDNA Reverse Transcription Kit (Life Technologies, Carlsbad, CA) according to manufacturer’s instructions.
Real-time PCR and fluorescence detection were performed using QuantStudio 12K Flex Real-Time PCR System (Life Technologies, Carlsbad, CA) following manufacturer’s instructions, using Custom TaqMan OpenArray Real-Time PCR Plates
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
CONTROL 1 WEEK
Ba-nr2
|
| Data processing |
Cq values from the 47 samples analyzed were exported for normalization using geNorm (qBasePLUS software (Biogazelle, Gent, Belgium)). Two genes showed the most stable expression (Stat6 and Itgb2) and were used for normalization.
|
| |
|
| Submission date |
Mar 21, 2018 |
| Last update date |
May 22, 2018 |
| Contact name |
Emma Carmelo |
| E-mail(s) |
ecarmelo@ull.edu.es
|
| Phone |
639648290
|
| Organization name |
University of La Laguna
|
| Department |
University Institute of Tropical Diseases and Public Health
|
| Street address |
C/ Astrofisico Francisco Sanchez s/n, Campus de Anchieta
|
| City |
La Laguna |
| State/province |
SANTA CRUZ DE TENERIFE |
| ZIP/Postal code |
38296 |
| Country |
Spain |
| |
|
| Platform ID |
GPL21778 |
| Series (2) |
| GSE112129 |
Transcriptional profiling of immune-related genes in Leishmania infantum-infected mice: identification of potential biomarkers of infection and progression of disease [MODEL] |
| GSE112139 |
Transcriptional profiling of immune-related genes in Leishmania infantum-infected mice: identification of potential biomarkers of infection and progression of disease |
|
