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Sample GSM306064 Query DataSets for GSM306064
Status Public on Dec 19, 2008
Title SG-84A_phenotype of EPC colony assay
Sample type RNA
 
Source name cultured EPC, baseline
Organism Homo sapiens
Characteristics baseline
Treatment protocol cells were harvested after 5 days.
Growth protocol Blood was collected into CPT tubes with Ficoll and sodium heparin for isolation of mononuclear cells, washed twice in PBS with 5% fetal bovine serum and re-suspended in media for endothelial progenitor-colony-forming assay.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cultured EPC with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
Label biotin
Label protocol Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
Scan protocol Affymetrix GeneChip scanner
Description EPC sampled from blood and cultured
Data processing Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation
 
Submission date Jul 17, 2008
Last update date Aug 28, 2018
Contact name Richard Cannon
E-mail(s) cannonr@nih.gov
Phone 301-496-9895
Organization name NIH
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-1454
Country USA
 
Platform ID GPL570
Series (1)
GSE12155 Characterization of a Colony Assay Used to Investigate Endothelial Progenitor Cells
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE S10 transformed data.

Data table
ID_REF VALUE
1552401_a_at 263.41153
1552588_a_at 19.205423
1552757_s_at 178.924728
1552829_at 68.559044
1552867_at 226.822098
1552974_at 14.624074
1552975_x_at 169.521881
1552976_at 200.190964
1553083_at 184.356339
1553241_at 33.134762
1553275_s_at 33.254482
1553310_at 113.365234
1553355_at 39.130123
1553398_at 135.874954
1553413_at 7.524949
1553439_at 75.154663
1553449_at 33.758266
1553462_at 21.925207
1553498_at 102.319893
1553536_at 146.731491

Total number of rows: 54675

Table truncated, full table size 1152 Kbytes.




Supplementary file Size Download File type/resource
GSM306064.CEL.gz 5.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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