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Sample GSM306075 Query DataSets for GSM306075
Status Public on Dec 19, 2008
Title SG-94B_phenotype of EPC colony assay
Sample type RNA
 
Source name cultured EPC, after 3 months of exercise
Organism Homo sapiens
Characteristics after 3 months of exercise
Treatment protocol cells were harvested after 5 days.
Growth protocol Blood was collected into CPT tubes with Ficoll and sodium heparin for isolation of mononuclear cells, washed twice in PBS with 5% fetal bovine serum and re-suspended in media for endothelial progenitor-colony-forming assay.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cultured EPC with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
Label biotin
Label protocol Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
Scan protocol Affymetrix GeneChip scanner
Description EPC sampled from blood and cultured
Data processing Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation
 
Submission date Jul 17, 2008
Last update date Aug 28, 2018
Contact name Richard Cannon
E-mail(s) cannonr@nih.gov
Phone 301-496-9895
Organization name NIH
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-1454
Country USA
 
Platform ID GPL570
Series (1)
GSE12155 Characterization of a Colony Assay Used to Investigate Endothelial Progenitor Cells
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE S10 transformed data.

Data table
ID_REF VALUE
1552401_a_at 273.114563
1552588_a_at 170.879929
1552757_s_at 50.978527
1552829_at 90.796486
1552867_at 139.106186
1552974_at 9.009484
1552975_x_at 12.474692
1552976_at 15.100122
1553083_at 36.747082
1553241_at 366.106873
1553275_s_at 152.733612
1553310_at 104.784332
1553355_at 11.328132
1553398_at 90.173676
1553413_at 29.301262
1553439_at 259.449951
1553449_at 22.371786
1553462_at 99.426476
1553498_at 13.312428
1553536_at 154.246353

Total number of rows: 54675

Table truncated, full table size 1149 Kbytes.




Supplementary file Size Download File type/resource
GSM306075.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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