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Sample GSM306078 Query DataSets for GSM306078
Status Public on Dec 19, 2008
Title SG-103A_phenotype of EPC colony assay
Sample type RNA
 
Source name cultured EPC, baseline
Organism Homo sapiens
Characteristics baseline
Treatment protocol cells were harvested after 5 days.
Growth protocol Blood was collected into CPT tubes with Ficoll and sodium heparin for isolation of mononuclear cells, washed twice in PBS with 5% fetal bovine serum and re-suspended in media for endothelial progenitor-colony-forming assay.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cultured EPC with an RNAqueous micro RNA isolation kit (Ambion, Austin, TX). Cells were lysed in buffer containing guanidinium thiocyanate.
Label biotin
Label protocol Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
Scan protocol Affymetrix GeneChip scanner
Description EPC sampled from blood and cultured
Data processing Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation
 
Submission date Jul 17, 2008
Last update date Aug 28, 2018
Contact name Richard Cannon
E-mail(s) cannonr@nih.gov
Phone 301-496-9895
Organization name NIH
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-1454
Country USA
 
Platform ID GPL570
Series (1)
GSE12155 Characterization of a Colony Assay Used to Investigate Endothelial Progenitor Cells
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE S10 transformed data.

Data table
ID_REF VALUE
1552401_a_at 348.755646
1552588_a_at 4.69283
1552757_s_at 83.870613
1552829_at 139.637619
1552867_at 169.237244
1552974_at 11.078462
1552975_x_at 74.262482
1552976_at 18.682997
1553083_at 101.262321
1553241_at 136.351639
1553275_s_at 60.253567
1553310_at 14.066208
1553355_at 11.819619
1553398_at 21.507978
1553413_at 5.711573
1553439_at 57.981205
1553449_at 54.769627
1553462_at 100.464272
1553498_at 119.265488
1553536_at 133.205704

Total number of rows: 54675

Table truncated, full table size 1142 Kbytes.




Supplementary file Size Download File type/resource
GSM306078.CEL.gz 5.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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