|
| Status |
Public on Jul 13, 2009 |
| Title |
10354 |
| Sample type |
RNA |
| |
|
| Source name |
Whole Blood
|
| Organism |
Homo sapiens |
| Characteristics |
Recipient Age: 74
Recipient Sex: M
Drug Regime: FK506 [Tacrolimus]
Donor Age: 35
Donor Sex: F
CAN Banff Score =1
CAN scores are representative of the severity of can (0-mild CAN; 1-moderate CAN, 2&3- severe CAN)
|
| Treatment protocol |
N/A
|
| Growth protocol |
Cells were not manipulated before RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol and purified on RNeasy Columns for PBMC and Whole Blood RNA was extracted using the PaxGene Blood RNA system and Globin Transcripts reduced uisng GlobinClear
|
| Label |
biotin
|
| Label protocol |
Ambion MessageAmp Biotin II Enhanced
|
| |
|
| Hybridization protocol |
Standard Affymetrix 49 Format Hybridization with 15mg of fragmented cRNA
|
| Scan protocol |
Affymetrix EukGE-WS2v4
|
| Description |
RNA from Whole Blood of patients with Chronic Allograft Nephropathy or Well Functioning Kidney Transplants
|
| Data processing |
RMAExpress
|
| |
|
| Submission date |
Jul 21, 2008 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Sunil Kurian |
| E-mail(s) |
smkurian@scripps.edu
|
| Phone |
858-784-7759
|
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular and Experimental Medicine
|
| Street address |
10550 N Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12187 |
Biomarkers for Early and Late Stage Chronic Allograft Nephropathy by Genomic Profiling of Peripheral Blood |
|
| Relations |
| Reanalyzed by |
GSE122511 |
