|
| Status |
Public on Jun 30, 2011 |
| Title |
tMLL04 |
| Sample type |
RNA |
| |
|
| Source name |
tMLL04
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: MLL translocation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. The integrity of the RNA was assessed using the RNA 6000 Nano assay kit on a Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Standard Affymetrix according to manufacturer's instructions.
|
| Scan protocol |
Standard Affymetrix according to manufacturer's instructions.
|
| Description |
Gene expression data from bone marrow
|
| Data processing |
Probeset intensities were extracted from CEL-files using GeneChip Operating Software (GCOS) (Affymetrix). Probe intensities were normalized. Differentially expressed genes between mixed lineage leukemia translocation (tMLL) and MLL-partial tandem duplication (MLL-PTD) subgroups were calculated using the Student t-test.
|
| |
|
| Submission date |
Jul 27, 2008 |
| Last update date |
Jun 30, 2011 |
| Contact name |
Hsi-Che Liu |
| E-mail(s) |
hsiche@mmh.org.tw
|
| Organization name |
Mackay Memorial Hospital
|
| Street address |
No. 92, Sec. 2, Chung-San North Rd.
|
| City |
Taipei |
| ZIP/Postal code |
10449 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE15013 |
Expression of HOXB genes is significantly different in acute myeloid leukemia with a partial tandem duplication of MLL vs. a MLL translocation: a cross-laboratory study |
|
