|
| Status |
Public on Oct 31, 2008 |
| Title |
Patient with CAD; patient group: control; CADi: 0 (d3918) |
| Sample type |
RNA |
| |
|
| Source name |
Homo sapiens;tissue: blood cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood cells
cohort: pooled
patient group: control
sex: m
age: 50
ethnicity: caucasian
CADi: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression analysis in patient with coronary artery disease (CADi>0) in comparison to control patients (CADi=0)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jul 30, 2008 |
| Last update date |
Jul 30, 2008 |
| Contact name |
Peter Grass |
| E-mail(s) |
peter.grass@novartis.com
|
| Phone |
+41 61 6961558
|
| Organization name |
Novartis
|
| Department |
DMP
|
| Lab |
QB
|
| Street address |
Klybeck Strasse
|
| City |
Basel |
| ZIP/Postal code |
4001 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE12288 |
Gene expression patterns in peripheral blood correlate with the extent of coronary artery disease |
|
