|
| Status |
Public on Sep 30, 2019 |
| Title |
14.5 R1 +HI_R1 |
| Sample type |
RNA |
| |
|
| Source name |
enzymatically disaggregated embryonic heart ventricles into single cells from Wt1GFPKI mice
|
| Organism |
Mus musculus |
| Characteristics |
stage of development: E14.5
|
| Treatment protocol |
Samples were FACS sorted and RNA lysis buffer was added post sorting to avoid mRNA changes
|
| Growth protocol |
cells were extracted from enzymatically disaggregated embryonic heart ventricles into single cells and used directly for mRNA studies.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Total RNA isolation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Austin, TX, U.S.A.) according to the manufacturer’s specifications with an input of 200 ng total RNA.
|
| |
|
| Hybridization protocol |
Hybridization and washing were performed according to the Illumina Manual “Direct Hybridization Assay Guide”
|
| Scan protocol |
Scanning was performed on the Illumina iScan (Illumina, Inc., San Diego, CA, U.S.A.).
|
| Description |
Wt1GFP++ Cells at day 14.5
|
| Data processing |
Image analysis and extraction of raw expression data was performed with Illumina GenomeStudio v2011.1 Gene Expression software with default settings (no background substraction and no normalization)
|
| |
|
| Submission date |
Apr 17, 2018 |
| Last update date |
Sep 30, 2019 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE113273 |
Identification of the gene profiling of Wt1GFP enriched epicardial cells at different stages of embryonic heart development [H] |
| GSE113276 |
Identification of the gene profiling of Wt1GFP expressing cardiomyocytes at different stages of embryonic heart development |
|
