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Status |
Public on Mar 31, 2020 |
Title |
Retinal microvessels, nondiabetic, biological rep6 |
Sample type |
RNA |
|
|
Source name |
Retinal microvessels
|
Organism |
Rattus norvegicus |
Characteristics |
diagnosis: non-diabetic tissue: Retina tissue compartment: Retinal microvessels Sex: male strain: Wistar
|
Treatment protocol |
Diabetes was induced with tail vein injection of Streptozotocin. These retinal and brain crysections were homogenated and centrifuged. The microvessels samples were captured by filtering the homogenate with nylon mesh.
|
Growth protocol |
The retinae and brain hemisphere from individual rat was cryosectioned.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was obtained with RNeasy Plus Micro kit (Qiagen, Hilden, Germany)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol from 20 ug total RNA
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|
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Hybridization protocol |
Following fragmentation, 5.5 ug of cRNA were hybridized for 16 hr at on the Genechip Fluidics Station 450 (Affymetrix)
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Scan protocol |
GeneChips were scanned using the Genechip Scanner 7G (Affymetrix)
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Data processing |
These raw CEL files were normalized using the Affymetrix® Expression Console Software (version 4.0, Affymetrix) and the adjusted intensity values were transformed to log2 format
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Submission date |
Apr 25, 2018 |
Last update date |
Apr 01, 2020 |
Contact name |
Youhai Li |
Organization name |
University Medical Center Groningen (UMCG)
|
Department |
Dept. Pathology and Medical Biology
|
Lab |
Medical Biology
|
Street address |
Hanzeplein 1
|
City |
Groningen |
State/province |
Groningen |
ZIP/Postal code |
EA11 |
Country |
Netherlands |
|
|
Platform ID |
GPL17117 |
Series (1) |
GSE113686 |
Responses of retinal and brain microvasculature to diabetes revealed by global expression profiling |
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