|
| Status |
Public on Mar 31, 2020 |
| Title |
Brain microvessels, nondiabetic, biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Brain microvessels
|
| Organism |
Rattus norvegicus |
| Characteristics |
diagnosis: non-diabetic
tissue: Brain
tissue compartment: Brain microvessels
Sex: male
strain: Wistar
|
| Treatment protocol |
Diabetes was induced with tail vein injection of Streptozotocin. These retinal and brain crysections were homogenated and centrifuged. The microvessels samples were captured by filtering the homogenate with nylon mesh.
|
| Growth protocol |
The retinae and brain hemisphere from individual rat was cryosectioned.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained with RNeasy Plus Micro kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol from 20 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cRNA were hybridized for 16 hr at on the Genechip Fluidics Station 450 (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 7G (Affymetrix)
|
| Data processing |
These raw CEL files were normalized using the Affymetrix® Expression Console Software (version 4.0, Affymetrix) and the adjusted intensity values were transformed to log2 format
|
| |
|
| Submission date |
Apr 25, 2018 |
| Last update date |
Apr 01, 2020 |
| Contact name |
Youhai Li |
| Organization name |
University Medical Center Groningen (UMCG)
|
| Department |
Dept. Pathology and Medical Biology
|
| Lab |
Medical Biology
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
EA11 |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE113686 |
Responses of retinal and brain microvasculature to diabetes revealed by global expression profiling |
|
