|
| Status |
Public on Sep 02, 2008 |
| Title |
Wild-type |
| Sample type |
SRA |
| |
|
| Source name |
wild-type
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
genotype: wild-type Ago1 pull-down
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ago1-associated RNAs were recovered from Flag-purified FLAG-Ago1 protein by phenol-chloroform extraction and EtOH precipitation
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
454 GS |
| |
|
| Description |
Argonaute-associated small RNAs were sequenced from wild-type fission yeast.
|
| Data processing |
We selected 454 reads with matches to the terminal 9 nucleotides of the 5' linker and the first 9 nucleotides of the 3' linker.
|
| |
|
| Submission date |
Aug 12, 2008 |
| Last update date |
May 15, 2019 |
| Contact name |
Noah Spies |
| Organization name |
Stanford University
|
| Department |
Pathology
|
| Lab |
Arend Sidow
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL9157 |
| Series (1) |
| GSE12416 |
TRAMP-mediated RNA surveillance prevents spurious entry of RNAs into the S. pombe siRNA pathway |
|
| Relations |
| SRA |
SRX001945 |
| BioSample |
SAMN02195244 |
