|
| Status |
Public on Dec 26, 2018 |
| Title |
liver_MCD_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
NASH mice with MCD diet
|
| Organism |
Mus musculus |
| Characteristics |
Sex: male
strain: C57BL/6
tissue: liver
|
| Treatment protocol |
MCD group were treated either with vehicle (1×PBS) or 450 mg/kg DDC via daily gavage for eight weeks.
|
| Growth protocol |
Eight-week-old male C57BL/6 mice were kept on MCD or MCS control diet for eight weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction by Trizol Reagent. RNA purity was checked by optical density of NanoDrop ND-1000 and agarose electrophoresis with the result of A260/A280≧1.8, A260/A230≧1.5, no gDNA contamination. RNA integrity was measured by Agilent RNA 6000 Nano Assay (RIN≧6).
|
| Label |
cy5
|
| Label protocol |
1 µg of total RNA was reverse-transcribed and amplified using MessageAmpTM aRNA Amplification Kit (Ambion). Indirect labeling the aa-UTP whereon by NHS-CyDye (Cy5, Amershan).
|
| |
|
| Hybridization protocol |
MOA2.1 arrays were pre-heat at 60°C for 10 mins, rehydrated by 100% ethanol following with deionized water. The slides were pre-hybridized with 5x SSPE, 0.1% SDS and 1% BSA at 42℃ for 2 hour. After the pre-hybridization, 5 µg Cy5-labeled aRNA was hybridize on MOA in the presentation of the Phalanx OneArray hybridization buffer in duplication.
|
| Scan protocol |
The arrays were scanned by GenePix 4000B scanner (635nm power 100 PMT 520 ; 532nm power 10, PMT 460) and quantify the fluoresence intensity.
|
| Data processing |
Data processing was performed and calculated by Rosetta Resolver (Rosetta Biosoftware). The probes <0 in all samples were filtered out, then normalized by median scaling method between technical replicates. Pair-wise comparisons between samples were calculated by Rosetta error model and presented by log2 ratio and p-value.
|
| |
|
| Submission date |
Apr 30, 2018 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Tianhui Liu |
| E-mail(s) |
liu_tianhui@163.com
|
| Organization name |
Beijing Friendship Hospital, Capital Medical University
|
| Street address |
No. 95 Yong-an Road, Xi-cheng District
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
| |
|
| Platform ID |
GPL15523 |
| Series (1) |
| GSE113843 |
Diethyldithiocarbamate, an anti-abuse drug, alleviates steatohepatitis and fibrosis in rodents |
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