|
| Status |
Public on Apr 30, 2023 |
| Title |
adjacent normal sample 9984 |
| Sample type |
RNA |
| |
|
| Source name |
human breast cancer adjacent tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Ambion, Austin, TX, USA), and clean-up was performed using chloroform and isopropanol.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified and purified using an Ambion Illumina RNA amplification kit (Ambion) to yield biotinylated cRNA according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The quality of hybridization and overall chip performance were monitored by visual inspection of both internal quality control checks and the raw scanned data. Raw data were extracted using the software provided by the manufacturer (Illumina GenomeStudio v2011.1 (Gene Expression Module v1.9.0)). Probes signal value was transformed by logarithm and normalized by quantile method.
|
| |
|
| Submission date |
Apr 30, 2018 |
| Last update date |
Apr 30, 2023 |
| Contact name |
Jong Hoon Park |
| E-mail(s) |
parkjh@sm.ac.kr
|
| Phone |
822-710-9414
|
| Organization name |
Sookmyung Women's university
|
| Lab |
Molecular Medicine Lab
|
| Street address |
Cheongpa-ro 47-gil 100, Yongsan-gu,
|
| City |
Seoul |
| ZIP/Postal code |
04310 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE113865 |
Genome-wide gene expression analysis of triple negative breast cancer tissue and matched normal tissue |
|
