|
| Status |
Public on Aug 21, 2008 |
| Title |
Endometrium, postmenopausal, estradiol + MPA, 29 |
| Sample type |
RNA |
| |
|
| Source name |
Endometrium from estradiol- and MPA-treated postmenopausal woman
|
| Organism |
Homo sapiens |
| Characteristics |
Postmenopausal female. Estradiol + MPA treated. Patient 29.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG 133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning procedures were carried out as described by Affymetrix, Santa Clara.
|
| Description |
Gene expression data. Endometrium from postmenopausal female (Klaassens et al., 2006, Hanifi-Moghaddam et al., 2007). Estradiol + medroxyprogesterone acetate treated. Patient 29.
|
| Data processing |
The data were analyzed with RMAExpress using Affymetrix default settings and were normalized according to the quantile method (Bolstad BM, Irizarry RA, Astrand M, Speed TP. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 2003;19:185-93). After normalization, the intensity values below 30 were set at 30.T
|
| |
|
| Submission date |
Aug 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Leen J Blok |
| E-mail(s) |
l.blok@erasmusmc.nl
|
| Phone |
31-10-7044111
|
| Fax |
31-10-7044365
|
| Organization name |
Erasmus University Medical Center
|
| Department |
Obstetrics & Gynecology
|
| Lab |
Gynecologic Oncology
|
| Street address |
Dr Molewaterplein 40
|
| City |
Rotterdam |
| ZIP/Postal code |
3000 CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12446 |
Endometrium of hormone-treated postmenopausal women |
|
| Relations |
| Reanalyzed by |
GSE119087 |
