|
| Status |
Public on Aug 31, 2008 |
| Title |
224 Day 3 siMut10 transfected biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cultured human melanoma cancer cell 224
|
| Organism |
Homo sapiens |
| Characteristics |
cell line
|
| Treatment protocol |
NRAS-MUT10 (siMut10) transfected cells from Day 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from cell culture using RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized, washed and stained using the Affymetrix Fluidics Station 400 according to Affymetrix standard procedures
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from cultured melanoma cancer cell line
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
|
| |
|
| Submission date |
Aug 14, 2008 |
| Last update date |
Aug 14, 2008 |
| Contact name |
Fei Huang |
| Organization name |
Bristol-Myers Squibb Co
|
| Street address |
P.O. Box 5400/HW3B2.02
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08543 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE12445 |
Expression data from melanoma cell lines 224 and BL transfected with siRNA-NRASQ61R |
|
