|
| Status |
Public on Jul 06, 2018 |
| Title |
Mung bean leaf |
| Sample type |
SRA |
| |
|
| Source name |
Leaf
|
| Organism |
Vigna radiata |
| Characteristics |
developmental stage: 2-week old
tissue: leaf
|
| Growth protocol |
Arabidopsis and mung bean were grown in a growth chamber or greenhouse with a light cycle of 16 h light / 8 h dark, at 22 to 23 °C. Pigeon pea plants were grown in a greenhouse with a light cycle of 16 h light / 8 h dark, at 24 to 28 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples in this study were purified from plant materials using PureLink Plant RNA Reagent.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The adapter sequences were trimmed from raw sequence reads, and then the small RNA sequences were trimmed to the lengths between 15 and 34 nucleotides. Small RNA reads were mapped to the Pigeon pea, mung bean, and Arabidopsis genomes.
Genome_build: Pigeon pea Cc1.0; mung bean Vr1.0; Arabidopsis thaliana TAIR10.
Supplementary_files_format_and_content: Tag count file
|
| |
|
| Submission date |
May 04, 2018 |
| Last update date |
Jul 07, 2018 |
| Contact name |
Blake C. Meyers |
| E-mail(s) |
bmeyers@danforthcenter.org
|
| Phone |
314-587-1422
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Meyers lab
|
| Street address |
975 N Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
| |
|
| Platform ID |
GPL24965 |
| Series (1) |
| GSE114075 |
Biogenesis of a young, 22-nt microRNA in Phaseoleae species by precursor-programmed uridylation |
|
| Relations |
| BioSample |
SAMN09078331 |
| SRA |
SRX4045480 |
