|
| Status |
Public on Jun 30, 2021 |
| Title |
mPFC_social defeat_susceptible_Right |
| Sample type |
RNA |
| |
|
| Source name |
mouse right mPFC of susceptible of social defeat mice
|
| Organism |
Mus musculus |
| Characteristics |
region: medial prefrontal cortex
tissue: brain
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of each 7 representative mice was prepared by Trizol method then further purified by RNAeasy kit (Qiagen, USA). The left and right mPFC RNA of susceptible, resilient and control mice was used for microarray analysis with the MouseRef-8_V2 Expression BeadChip (Illumina).
|
| Label |
biotin
|
| Label protocol |
Illumina
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
The prepared microarrays were scanned with an Illumina BeadArray reader
|
| Description |
sr1
|
| Data processing |
The prepared microarrays were preprocessed by the Illumina GenomeStudio software (version 1.0.6). Raw signals of at least two biological replicates per condition were transformed to log2 scale and normalized by using the Bioconductor lumi package. Differentially expressed genes (DEGs) were identified by using the Bioconductor limma package with an false discovery rate (FDR) adjusted p-value cutoff of 0.05.
|
| |
|
| Submission date |
May 09, 2018 |
| Last update date |
Jun 30, 2021 |
| Contact name |
Sujin Chae |
| E-mail(s) |
jinichae@gmail.com
|
| Organization name |
KAIST
|
| Street address |
291 Daehak-ro
|
| City |
Daejeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE114224 |
Hemispheric lateralization of gene expression profile in stressed mice |
|
