|
| Status |
Public on Dec 31, 2018 |
| Title |
HH1_early_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Fertilized egg, Chicken HH1 Early (0.5hour)
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Area pelucida (AP) region
developmental stage: HH1 Early (0.5 hour)
|
| Treatment protocol |
The area pelucida (AP) region of 0.5h, 5h, 9h and 14h incubated embryos, containing both the epiblast and hypoblast was cut out in Pannett-Compton solution. Isolated area pellucida tissues were collected and used for RNA isolation.
|
| Growth protocol |
Fertilized hens’ eggs were incubated at 38.5°C for 0.5h, 5h, 9h or 14h in a humid incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Two independent sets of total RNA from pooled AP samples of the different time points were isolated using Qiagen RNeasy kit. RNA concentrations were measured and equalized, and RNA quality was checked on gel. 100ng of total RNA were used for each array.
|
| Label |
biotin
|
| Label protocol |
cDNA samples were subjected to the two-cycle target labeling procedure for biotin labeling by in vitro transcription (Affymetrix).
|
| |
|
| Hybridization protocol |
RNA was subsequently fragmented and hybridized to the GeneChip according to manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Epiblast-2 0.5h-1
Gene expression data from (potential) primitive streak region in chicken HH1 early (0.5hour), biological replicate 1.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and expression values are quantified with the RMA method (Bioconductor 3.6).
|
| |
|
| Submission date |
May 15, 2018 |
| Last update date |
Dec 31, 2018 |
| Contact name |
Cantas Alev |
| Organization name |
Kyoto University
|
| Department |
Institute for the Advanced Study of Human Biology (ASHBi)
|
| Lab |
Alev-Lab
|
| Street address |
Medical Faculty Bldg. B, Yoshida-Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE114476 |
Mesenchymal-Epithelial Transition Regulates Initiation of Pluripotency Exit before Gastrulation |
|
