|
| Status |
Public on Jan 31, 2019 |
| Title |
WM1617 |
| Sample type |
genomic |
| |
|
| Source name |
melanoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
origin: metastatic melanoma tumor (primary pair: WM278)
braf mutation status: V600E
|
| Treatment protocol |
Human melanoma cell lines were switched to a medium containing 5 µM PLX4720 and cultured. The surviving cells were fed a medium containing PLX4720 every 3 days until they reached 80% confluence
|
| Growth protocol |
Human melanoma cell lines were maintained in RPMI 1640 medium supplemented with 10% foetal bovine serum, 300 mg/l L-glutamine and antibiotics.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was purified using Intron G-Spin Genomic DNA Extraction kit.
|
| Label |
biotin
|
| Label protocol |
Labeling, hybridization and image scan were performed according to the recent laboratory protocol of UD-GenoMed Medical Genomic Technologies Ltd.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Affymetrix Genotyping Console was used for primary analysis. Copy number values were extracted and converted from CEL files into signal intensities using apt-copynumber-cyto 1.14.4 software, and CYCHP files were generated by Chromosome Analysis Suite (ChAS) 2.0 software according to the current protocol of UD-GenoMed Medical Genomic Technologies Ltd. ChAS 2.0 and Nexus 6.1 Copy Number Discovery softwares were used for further analysis.
|
| |
|
| Submission date |
May 15, 2018 |
| Last update date |
Jan 31, 2019 |
| Contact name |
Viktoria Koroknai |
| E-mail(s) |
koroknai.viktoria@med.unideb.hu
|
| Organization name |
University of Debrecen
|
| Department |
Department of Public Health and Epidemiology
|
| Street address |
26/B Kassai str.
|
| City |
Debrecen |
| ZIP/Postal code |
4028 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE114488 |
CNV patterns in BRAFi resistant melanoma cell lines |
|
