|
| Status |
Public on May 12, 2020 |
| Title |
12-0358p15Ctrl |
| Sample type |
RNA |
| |
|
| Source name |
Patient-derived GBM cell line_MTAP WT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 12-0358
cell type: patient-derived primary glioblastoma cell line
genotype/variation: MTAP WT
treated with: vehicle control NaOH (1:1000 dilution)
|
| Treatment protocol |
none
|
| Growth protocol |
Patient derived GBM cell lines were grown in human Neural Stem Cell media following the NeuroCult NS-A Proliferation Protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Zymo Quick RNA prep kit
|
| Label |
biotin
|
| Label protocol |
Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE). Hybridization targets were prepared with the affymetrix GeneChip 3' IVT Plus Kit from total RNA.
|
| |
|
| Hybridization protocol |
Product generated from the label protocol was hybridized onto the Affymetrix GeneChip Human Genome 2.0 plus array in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450 according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA).
|
| Scan protocol |
Arrays were scanned with Affymetrix GeneChip® Scanner 7G according to Affymetrix GeneChip protocols
|
| Description |
Control
|
| Data processing |
Data was initially processed using the Affymetrix Expresson Console and Affymetrix Transcriptome Analysis Console v3.0 software
|
| |
|
| Submission date |
May 24, 2018 |
| Last update date |
May 13, 2020 |
| Contact name |
Landon J Hansen |
| E-mail(s) |
landon.hansen@duke.edu
|
| Organization name |
Duke University School of Medicine
|
| Street address |
203 Research Drive, MSRB1 Rm 171
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE114867 |
Expression data from patient-derived primary glioblastoma cell lines |
|
