|
| Status |
Public on Aug 31, 2018 |
| Title |
Participant 7 |
| Sample type |
RNA |
| |
|
| Source name |
Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
recovery: Good
tissue: plasma
delta-arat: 18
days post-stroke: 12
age: 83
gender: F
ischemic or hemorrhagic stroke: Ischemic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from 200uL plasma using Qiagen miRNeasy Serum/Plasma Kit
|
| Label |
FAM
|
| Label protocol |
RT performed using 33ng total RNA with Applied Biosystems Megaplex RT Primers, Human Pool A and B v3.0, followed by 12 cycles preamplification. cDNAs loaded onto Taqman Array Human MicroRNA A+B Cards, v3.0, Applied Biosystems. qRT-PCR performed on 7900HT rtPCR System (Applied Biosystems)
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
SAMPLE 7
|
| Data processing |
miRNA expression values were normalized with log transformation followed by quantile normalization
normalized file reports normalized signal (against housekeeping genes)
Fold change file reports good vs. poor recovery ratios
|
| |
|
| Submission date |
May 24, 2018 |
| Last update date |
Nov 28, 2023 |
| Contact name |
Matthew Edwardson |
| E-mail(s) |
mae97@georgetown.edu
|
| Phone |
2026872184
|
| Organization name |
Georgetown University
|
| Department |
Neurology
|
| Street address |
4000 Reservoir Rd NW, Building D, Room 207
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20007 |
| Country |
USA |
| |
|
| Platform ID |
GPL18942 |
| Series (1) |
| GSE114897 |
Real-time quantitative PCR analysis of human stroke patients recovering from upper extremity hemiparesis |
|
