|
| Status |
Public on Dec 31, 2018 |
| Title |
duodenum_Glu_negative_4665 |
| Sample type |
SRA |
| |
|
| Source name |
duodenum
|
| Organism |
Mus musculus |
| Characteristics |
tissue: duodenum
strain: GluVenus
cell population: negative
|
| Growth protocol |
mice were grown on a 12h dark/light cycle with ad libitum access to water and standard chow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
duodenum epithelium was single-cell digestedand FACS sorted for positive cells and similar number of negative cells. RNA was extracted using microplus RNeasy kit (Qiagen) and quality checked on a picochip bioanalyzer (RIN >7)
Library was constructed with 2ng of total RNA using the Ovation RNAseq system v2 kit and the Ovation Rapid DR multiplex 1-96 kit (Nugen)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
duodenum_Glu_negative_4665
|
| Data processing |
data was demultiplexed using the Casava pipeline
data was mapped to the mouse genome GRCm38 using Tophat v2.1.0 and raw fragment counts generated with Cufflinks v2.2.1
Genome_build: GRCm38
Supplementary_files_format_and_content: mouse_duo_Neurod1_GluVenus_raw_count.txt is a tab delimited file of number of counts for ensembl gene ids of each biological sample
|
| |
|
| Submission date |
May 25, 2018 |
| Last update date |
Dec 31, 2018 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE114913 |
Mouse duodenal enteroendocrine transcriptome |
|
| Relations |
| BioSample |
SAMN09259622 |
| SRA |
SRX4124272 |
