|
| Status |
Public on Mar 27, 2019 |
| Title |
Mammary gland cow nb 1015 restricted group |
| Sample type |
RNA |
| |
|
| Source name |
Mammary gland biopsy Restricted
|
| Organism |
Bos taurus |
| Characteristics |
physiological stage: early lactation stage, multiparous.
breed: Holstain cow
diet: Restricted
|
| Treatment protocol |
At 24 ± 3 d in milk, multiparous cows were either allowed to continue ad libitum intake of a lactation diet (CON, n = 6), or the ration was diluted with barley straw (48% DM) for 4 d (RES, n = 6). On d 3, one healthy rear mammary quarter was infused with 50 µg of LPS. mammary gland biopsies were performed 24 h after LPS challenge
|
| Growth protocol |
Twelve cows were studied at early lactation stage,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared through homogenization of approximately 50 mg of mammary gland tissue according to manufacture protocol of mirVana miRNA extraction kit (Invitrogen Life Technologies, Cergy Pontoise, France)
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA extracted were amplified and fluorescently labelled to produce Cy3 cRNA using the Low Input Quick Amp Labeling one color Kit (Agilent, USA) in the presence of spike-in one color control.
|
| |
|
| Hybridization protocol |
After purification (Qiagen RNeasy Mini Kit), 1,65 µg of Cy3 labeled cRNA were hybridized onto G2519F-023647 Bovine (V2) Gene Expression Microarray, 4x44K (Agilent, USA) with hybridization buffer (Agilent Gene Expression Hybridization Kit) according to the manufacturer’s instructions. After 17h of hybridization, slides were washed with the Gene Expression Wash Pack.
|
| Scan protocol |
Microarrays were scanned with Agilent G2505 scanner.
|
| Description |
Biological replicate 1 of 6
|
| Data processing |
Data were extracted with Feature Extraction 11,0 software (Agilent, USA) (no background subtraction).
|
| |
|
| Submission date |
May 29, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Celine BOBY |
| Organization name |
INRA
|
| Street address |
Centre INRA Auvergne-Rhône-Alpes
|
| City |
Saint Genes Champanelle |
| ZIP/Postal code |
63122 |
| Country |
France |
| |
|
| Platform ID |
GPL11648 |
| Series (1) |
| GSE114975 |
Mammary Gland transcriptome modifications by nutrient restriction in early lactation Holstein cows challenged with intramammary lipopolysaccharide |
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