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Status |
Public on Dec 10, 2008 |
Title |
CD34+_BM_2 |
Sample type |
RNA |
|
|
Source name |
Stem/progenitor hematopoietic cells CD34+ from bone marrow (BM)
|
Organism |
Homo sapiens |
Characteristics |
type: primary cells from healthy donors
|
Growth protocol |
Human CD34+ cells were purified from bone marrow blood samples: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep Human CD34 Positive Selection Kit, StemCell Technologies).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total cellular RNA was isolated by means of RNeasy Mini Kit (Qiagen, Valencia, CA) following manufacturer's recommendations.
|
Label |
biotin
|
Label protocol |
RNA samples from cells of different donors were pooled to obtain 5 μg of total cellular RNA that were used for target synthesis according to the standard one-cycle Affymetrix protocol, supplied by the manufacturer (Affymetrix, Santa Clara, CA).
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner GCS3000.
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Description |
Stem/progenitor hematopoietic cells CD34+ from bone marrow (BM)
|
Data processing |
The data were analyzed using Bioconductor packages and RMA pre-processing procedure
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|
|
Submission date |
Sep 16, 2008 |
Last update date |
Sep 17, 2008 |
Contact name |
Francesco Ferrari |
E-mail(s) |
ferrari.francesco@unimore.it
|
Phone |
+39(0)592055512
|
Fax |
+39(0)592055410
|
URL |
http://www.xlab.unimo.it
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Organization name |
University of Modena and Reggio Emilia
|
Department |
Department of Biomedical Sciences
|
Lab |
Experimental genomics & transcriptomics laboratory
|
Street address |
Via G. Campi 287
|
City |
Modena |
ZIP/Postal code |
41100 |
Country |
Italy |
|
|
Platform ID |
GPL96 |
Series (2) |
GSE12803 |
Gene expression in human myeloid cells |
GSE12837 |
Gene expression in human myeloid cells. |
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