|
| Status |
Public on Sep 09, 2010 |
| Title |
Buffycoat_patient41_time5 |
| Sample type |
RNA |
| |
|
| Source name |
Buffycoat
|
| Organism |
Homo sapiens |
| Characteristics |
Gender:Male,Ethnicity:Caucasian,Age:33,VAP day:5
|
| Growth protocol |
Samples from each patient were enriched using a previously described (monocyte or Neutrophil) enrichment protocol or Buffy coat protocol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Modiefied Qiagen Rneasy protocol
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip IVT labeling
|
| |
|
| Hybridization protocol |
GeneCip IVT hyb. Affymetrix EukGE-WSv5 wash and stain protocol
|
| Scan protocol |
Affymetrix GeneChip using GCOS.
|
| Description |
Gene expression data (Affymetrix GeneChip Human Genome U133 Plus 2.0 Array)
|
| Data processing |
The data were analyzed with RMA using default analysis settings and log2 scaling.
|
| |
|
| Submission date |
Sep 18, 2008 |
| Last update date |
Sep 09, 2009 |
| Contact name |
J Perren Cobb |
| E-mail(s) |
jpcobb@partners.org
|
| Phone |
314-362-1264
|
| Fax |
314-362-7474
|
| URL |
http://www.cia.wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Lab |
Cellular Injury and Adaptation Laboratory
|
| Street address |
Campus Box 8109, 660 South Euclid Avenue
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
|
