|
| Status |
Public on Sep 25, 2008 |
| Title |
JPA528 |
| Sample type |
RNA |
| |
|
| Source name |
JPA528
|
| Organism |
Homo sapiens |
| Characteristics |
Juvenile pilocytic astrocytoma
Age: 3 Sex: F
Location: cerebellum
Histopathology: focal vascular proliferation, Ki-67 (3-5%)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by RNeasy was performed according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner Model G2500
|
| Description |
Gene expression from juvenile pilocytic astrocytoma
|
| Data processing |
The data were analyzed using GCOS1.4
|
| |
|
| Submission date |
Sep 23, 2008 |
| Last update date |
Sep 26, 2008 |
| Contact name |
Kwong-Kwok Wong |
| E-mail(s) |
kkwong@mdanderson.org
|
| Phone |
713-792-0229
|
| Organization name |
UT M. D. Anderson Cancer Center
|
| Department |
Gynecologic Oncology and Reproductive Medicine
|
| Street address |
T4-3900, 1515 Holcombe Boulevard
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE12907 |
Expression Analysis of Juvenile Pilocytic Astrocytomas by Oligonucleotide Microarray Reveals Two Potential Subgroups |
|
